Role of Aminoacyl-Transfer Ribonucleic Acid in the Regulation of Ribonucleic Acid Synthesis in Escherichia coli
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چکیده
منابع مشابه
Stimulation of ribonucleic acid synthesis by chloramphenicol in a rel + aminoacyl-transfer ribonucleic acid synthetase mutant of Escherichia coli.
Escherichia coli strain 9D3 possesses a highly temperature-sensitive valyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.9). Since 9D3 is a rel(+) strain, it cannot carry out net RNA synthesis at high temperature. A 100-mug amount of chloramphenicol (CAP) per ml added in the absence of valine cannot stimulate RNA synthesis. Either 300 mug of CAP or 100 mug of CAP plus 50 mug of valine p...
متن کاملRegulation of ribonucleic acid synthesis in Escherichia coli during diauxie lag: accumulation of heterogeneous ribonucleic acid.
The synthesis of ribonucleic acid (RNA) and of protein in Escherichia coli during glucose-lactose diauxie lag have been examined. The rate of RNA synthesis is about 7%, of the corresponding rate during exponential growth and the rate of protein synthesis 10 to 15%. Inhibition of RNA synthesis occurs to the same extent in both rel and rel(+) strains. The RNA which accumulates during 20 min in di...
متن کاملProtein and Ribonucleic Acid Synthesis in a Mutant of Escherichia Coli with an Altered Aminoacyl Ribonucleic Acid Synthetase.
At present it is thought that adenosine triphosphate-mediated activation of the ol-carboxyl group of amino acids is a necessary step in the synthesis of at least some bacterial proteins. Evidence for the obligatory role of this activation has been available mainly from studies in vitro of protein synthesis (e.g. Simpson (2)), but a mutant of Escherichia coli has recently been isolated (1) which...
متن کاملRegulation of synthesis of the aminoacyl-transfer ribonucleic acid synthetases for the branched-chain amino acids of Escherichia coli.
The regulation of synthesis of valyl-, leucyl-, and isoleucyl-transfer ribonucleic acid (tRNA) synthetases was examined in strains of Escherichia coli and Salmonella typhimurium. When valine and isoleucine were limiting growth, the rate of formation of valyl-tRNA synthetase was derepressed about sixfold; addition of these amino acids caused repression of synthesis of this enzyme. The rate of sy...
متن کاملPreparation of Polyribosome Aminoacyl-Transfer Ribonucleic Acid
A procedure for preparing polyribosome aminoacyl-tRNA free from contamination by supernatant aminoacyl-tRNA and free amino acids is described. Important features of the procedure are the use of acidic buffers to help protect the amino acid-tRNA linkage and the inclusion ofsodium dodecyl sulphate, to inhibit ribonuclease activity. The specific radioactivity of polyribosome aminoacyl-tRNA is high...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1965
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.90.6.1624-1631.1965